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Nauka > Sequencing > Standard starters

Standard starters

Universal primers delivered by Genomed, free of charge.
(ref: Addgene Molecular Biology Reference)

id	name	sequence 5' - 3'	Tm
1	35Spromoter	CTATCCTTCGCAAGACCCTTC	54.4°C
2	3AOX1	GCAAATGGCATTCTGACATCC	52.4°C
3	5AOX1	GACTGGTTCCAATTGACAAGC	52.4°C
4	AC5	ACACAAAGCCGCTCCATCAG	53.8°C
5	ACYCDuetUP1	GGATCTCGACGCTCTCCCT	55.4°C
6	Alpha-factor	TACTATTGCCAGCATTGCTGC	52.4°C
7	Amp-R	ATAATACCGCGCCACATAGC	51.8°C
8	AUG1Forward	CAATTTACATCTTTATTTATTAACG	46.2°C
9	AUG1Reverse	GAAGAGAAAAACATTAGTTGGC	49.2°C
10	BGHrev	TAGAAGGCACAGTCGAGG	50.3°C
11	Bglob-intron-F	CTGGTCATCATCCTGCCTTT	51.8°C
12	Bglob-intron-R	TTTGCCCCCTCCATATAACA	49.7°C
13	Bglob-pA-R	TTTTGGCAGAGGGAAAAAGA	47.7°C
14	BRCA1_11BF	CAAGGAGCCAACATAACAGATG	53.0°C
15	BRCA1_11HR	GATGACGTCCTAGCTGTGTGAAG	57.1°C
16	CAT-R	GCAACTGACTGAAATGCCTC	51.8°C
17	CMVForward	CGCAAATGGGCGGTAGGCGTG	60.2°C
18	CRE-R	GCAAACGGACAGAAGCATTT	49.7°C
19	CYC1	GCGTGAATGTAAGCGTGAC	51.1°C
20	DsRed1-C	AGCTGGACATCACCTCCCACAACG	60.8°C
21	DsRed1-N	GTACTGGAACTGGGGGGACAG	58.3°C
22	EBVReverse	GTGGTTTGTCCAAACTCATC	49.7°C
23	EF-1aForward	TCAAGCCTCAGACAGTGGTTC	54.4°C
24	EGFP-C	CATGGTCCTGCTGGAGTTCGTG	58.6°C
25	EGFP-N	CGTCGCCGTCCAGCTCGACCAG	64.2°C
26	EXFP-R	GTCTTGTAGTTGCCGTCGTC	53.8°C
27	fD1	AGAGTTTGATCCTGGCTCAG	51.8°C
28	forpMal	CGATGAAGCCCTGAAAGAC	51.1°C
29	GAL1	AATATACCTCTATACTTTAACGTC	48.8°C
30	Gal10pro-F	GGTGGTAATGCCATGTAATATG	51.1°C
31	GAL4-AD	TACCACTACAATGGATGATGT	48.5°C
32	GAL4-BD	TCATCGGAAGAGAGTAGTAACA	51.1°C
33	Gal4N-term	GAGTAGTAACAAAGGTCAA	44.6°C
34	GFP-F	GGTCCTTCTTGAGTTTGTAAC	50.5°C
35	GFP-R	CCATCTAATTCAACAAGAATTGGGACAAC	57.3°C
36	GPDpro-F	CGGTAGGTATTGATTGTAATTCTG	52.3°C
37	gt7g_mk	GTAATACGACTCACTATAGGG	50.5°C
38	gT7_mk	GTAATACGACTCACTATAGG	47.7°C
39	GW-3	GCATGATGACCACCGATATG	51.8°C
40	GW-5	AATCTCGCCGGATCCTAACT	51.8°C
41	H1	TCGCTATGTGTTCTGGGAAA	49.7°C
42	HA-R	TCTGGGACGTCGTATGGGTA	53.8°C
43	HAT	GAGGAGCACGCTCATGCCCAC	60.2°C
44	hGH-PA-R	CCAGCTTGGTTCCCAATAGA	51.8°C
45	hrGFP-R	TCCCCGAGTACCACTTCATC	53.8°C
46	hUBCpro-F	TGAAGCTCCGGTTTTGAACT	49.7°C
47	IRES-F	TGGCTCTCCTCAAGCGTATT	51.8°C
48	IRES-R	CCTCACATTGCCAAAAGACG	51.8°C
49	L4440	AGCGAGTCAGTGAGCGAG	52.6°C
50	LacI-R	GGCATACTCTGCGACATCGT	53.8°C
51	LacZ-R	GACAGTATCGGCCTCAGGAA	53.8°C
52	LexA	CGTCAGCAGAGCTTCACCATTG	56.7°C
53	LKO15	GACTATCATATGCTTACCGT	47.7°C
54	LNCX	AGCTCGTTTAGTGAACCGTCAGATC	57.7°C
55	Luc-F	AGTCAAGTAACAACCGCGA	48.9°C
56	LucNrev	CCTTATGCAGTTGCTCTCC	51.1°C
57	M13(-40)	GTTTTCCCAGTCACGAC	47.1°C
58	M13fwd	GTAAAACGACGGCCAGT	47.1°C
59	M13pUCf	CCCAGTCACGACGTTGTAAAACG	57.1°C
60	M13pUCr	AGCGGATAACAATTTCACACAGG	53.5°C
61	M13rev	CAGGAAACAGCTATGAC	44.6°C
62	malEprimer	GGTCGTCAGACTGTCGATGAAGCC	60.8°C
63	MBP-F	GATGAAGCCCTGAAAGACGCGCAG	60.8°C
64	MMLV-F	ATCAGTTCGCTTCTCGCTTC	51.8°C
65	mPGK-F	CATTCTGCACGCTTCAAAAG	49.7°C
66	MSCV	CCCTTGAACCTCCTCGTTCGACC	60.6°C
67	MSCV-rev	CAGCGGGGCTGCTAAAGCGCATGC	64.2°C
68	MT1-F	GCTGTCCTCTAAGCGTCACC	55.9°C
69	MTForward	CATCTCAGTGCAACTAAA	43.5°C
70	mU6-F	CAGCACAAAAGGAAACTCACC	52.4°C
71	Myc	GCATCAATGCAGAAGCTGATCTCA	55.7°C
72	Neo-F	CGTTGGCTACCCGTGATATT	51.8°C
73	Neo-R	GCCCAGTCATAGCCGAATAG	53.8°C
74	Nmt1-F	GCAATGTGCAGCGAAACTAA	49.7°C
75	NOS-F	GCGTTCAAAAGTCGCCTAAG	51.8°C
76	OpIE2Forward	CGCAACGATCTGGTAAACAC	51.8°C
77	pACYC-F	TGAAGTCAGCCCCATACGAT	51.8°C
78	pAd-CMV	GCTAGAGATCTGGTACCGTC	53.8°C
79	pBABE3	ACCCTAACTGACACACATTCC	52.4°C
80	pBABE5	CTTTATCCAGCCCTCAC	47.1°C
81	pBADForward	ATGCCATAGCATTTTTATCC	45.6°C
82	pBADReverse	GATTTAATCTGTATCAGG	41.2°C
83	pBluescriptKS	TCGAGGTCGACGGTATC	49.5°C
84	pBluescriptSK	TCTAGAACTAGTGGATC	42.2°C
85	pBMN5	GCTTGGATACACGCCGC	51.9°C
86	pBRforBam	CTTGGAGCCACTATCGAC	50.3°C
87	pBRforEco	AATAGGCGTATCACGAGGC	51.1°C
88	pBRrevBam	GGTGATGTCGGCGATATAGG	53.8°C
89	pCasper-F	GGGTTTTATTAACTTACAT	40.3°C
90	pcDL-F	GTTGCCTTTACTTCTAGGCCT	52.4°C
91	pCMV5Fwd	CGTTGACGCAAATGGGCGG	55.4°C
92	pCMV5Rev	CCTCCACCCCATAATATTATAGAAGGACAC	60.3°C
93	pENTR-F	CTACAAACTCTTCCTGTTAGTTAG	52.3°C
94	pENTR-R	ATGGCTCATAACACCCCTTG	51.8°C
95	pET_ter	TGCTAGTTATTGCTCAGCGG	51.8°C
96	pET_up	GATGCGTCCGGCGTAGA	51.9°C
97	pFastBacF	TATTCCGGATTATTCATACCGTC	51.7°C
98	pGEX3	CCGGGAGCTGCATGTGTCAGAGG	62.4°C
99	pGEX5	GGGCTGGCAAGCCACGTTTGGTG	62.4°C
100	pGP704-R	AACAAGCCAGGGATGTAACG	51.8°C
101	pHybLexReverse	GAGTCACTTTAAAATTTGTATACAC	49.5°C
102	pJET12-F	CGACTCACTATAGGGAGAGCGGC	60.6°C
103	pJET12-R	AAGAACATCGATTTTCCATGGCAG	54.0°C
104	pLTet-F	ACTGAGCACATCAGCAGGAC	53.8°C
105	pLXSN5	CCCTTGAACCTCCTCGTTCGACC	60.6°C
106	pMRB101-F	AAGATGCAGGCAGCTGAGTT	51.8°C
107	pMT2-F	TTGCCTTTCTCTCCACAGGT	51.8°C
108	pMX-S1811	GACGGCATCGCAGCTTGGATACAC	60.8°C
109	Polyhedrinforwa	AAATGATAACCATCTCGC	43.5°C
110	Polyhedrinrever	GTCCAAGTTTCCCTG	41.9°C
111	pQEpromoter	CCCGAAAAGTGCCACCTG	52.6°C
112	pQErevers	GTTCTGAGGTCATTACTGG	48.9°C
113	pREPForward	GCTCGATACAATAAACGCC	48.9°C
114	pRS-marker	CGGCATCAGAGCAGATTGTA	51.8°C
115	Pry1	CTTAGCATGTCCGTGGGGTTTGAAT	57.7°C
116	pTrcHisForward	GAGGTATATATTAATGTATCG	44.6°C
117	pTrcHisReverse	GATTTAATCTGTATCAGG	41.2°C
118	pZIP	TCCTTTCCAGCGAGGTTCTA	51.8°C
119	RCAS-F	ACATGGGTGGTGGTATAGCGCTTGCG	62.7°C
120	RevLR	CACTTTGTACAAGAAAGCTGG	50.5°C
121	Rluc-F	CCAGGATTCTTTTCCAATGC	49.7°C
122	rP2	ACGGCTACCTTGTTACGACTT	52.4°C
123	RVprimer3	CTAGCAAAATAGGCTGTCCC	51.8°C
124	SFFV-F	ATTGATTGACTGCCCACCTC	51.8°C
125	SP6	ATTTAGGTGACACTATAG	41.2°C
126	SV40-spliceR	CACAAAGATCCGGACCAAAG	51.8°C
127	SV40pA-R	GAAATTTGTGATGCTATTGC	45.6°C
128	SV40pro-F	TATTTATGCAGAGGCCGAGG	51.8°C
129	T3	ATTAACCCTCACTAAAGGGA	47.7°C
130	T7	TAATACGACTCACTATAGG	44.6°C
131	T7g	GTAATACGACTCACTATAGGG	50.5°C
132	T7ter	GCTAGTTATTGCTCAGCGG	51.1°C
133	T7term	CTAGTTATTGCTCAGCGG	48.0°C
134	Tacpromoter	GAGCGGATAACAATTTCACACAGG	55.7°C
135	Tet-R	GGCGAGTTTACGGGTTGTTA	51.8°C
136	TK-pA-R	TTGTCTCCTTCCGTGTTTCA	49.7°C
137	Tn7-end	GGGGTGGAAATGGAGTTTTT	49.7°C
138	TRC-F	CAAGGCTGTTAGAGAGATAATTGGA	54.4°C
139	Ubx-F	AACTCGTACTTTGAACAGGC	49.7°C
140	V5Reverse	ACCGAGGAGAGGGTTAGGGAT	56.3°C
141	WPRE-R	CATAGCGTAAAAGGAGCAACA	50.5°C
142	XBG-R	GACTCCATTCGGGTGTTC	50.3°C
143	XEF1a	TTTCGCCCTAACTTCGTGAT	49.7°C
144	XpressForward	TATGGCTAGCATGACTGGT	48.9°C

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