Offer and prices
We offer the synthesis of DNA and RNA oligos, fluorescent and other oligonucleotide modifications as well as purification using the HPLC method. DNA oligonucleotides:
RNA oligonucleotides: Delivery: DNA and RNA oligos are delivered in a lyophylised form along with an information concerning the quantity in which a given lyophylisate should be resuspended to obtain 100 µM; concentration. We advise 10 mM Tris pH 7.5. when resuspending DNA oligos. Orders: Please order online by filling out the form. If you encounter any problem while ordering, please do it by e-mail. Order processing time:
Prices are listed in the tables below. With the help of the oligonucleotides ordering form it is possible to calculate the cost of an oligonucleotide. standard oligonucleotidesNet prices (PLN), minimum yield and offered synthesis scale of standard oligonucleotides: 1 Standard desalting consists in ethanol precipitation. It guarantees a level of purification sufficient for standard applications (PCR reaction and sequencing) 2 For oligonucleotides longer than 80 bp price is 110% higher. For oligonucleotides longer than 90 bp price is 120% higher. For oligonucleotides shorter than 15 bp price calculated as for 15-mer.. 3 Guaranteed yields apply to a 20-mer +/- 20%. For oligos longer than 33 bases minimum yields cannot be guaranteed. 4 0.02 µmol scale only for oligos up to 33 bases. A higher scale of synthesis - please contact us Wobble - zasada zdegenerowana na 3' końcu - 54 PLN antisense oligonucleotides (PTOs)Net prices (PLN), minimum yield and offered synthesis scale of phosphorothioates (PTOs, S-oligos). Prices include HPLC- and cartridge-purification, if the oligo is a "Full-PTO"! For mixed Phosphosphorothioate/Phosphorodiester-Oligos (i. e. "end capping") cartridge-purification will be charged at 65 PLN net price. We recommend this service
1 Guaranteed yields apply to a 20-mer +/- 20%. For oligos longer than 33 bases minimum yields cannot be guaranteed. A higher scale of synthesis - please contact us labelled oligonucleotidesGenomed offers labelling of oligos at 5' and 3' ends as well as internal modifications. If the type of labelling you are interested in is not to be found on the price list, please enquire. Synthesis of labelled oligonucleotides, offered scales, minmal amount and net prices: 1 The price of the standard oligonucleotide will be added 2 The prices for internal fluorescent modifications are valid for oligonucleotides <40 bases. For longer oligonucleotides please contact us. Internal modification with biotin requires 1050 PLN setup fee. This modification requires a "T" to be attached. For 5` thiol modification a set up fee of 540 PLN per order will be charged. Dual labelled probes
Prices (PLN netto), minimum yield and offered scale of synthesis of FRET oligonucleotides and Dual labeled Fluorogenic Probes: * HPLC purification and MALDI ToF quality control are included. LightCycler ProbesThe LightCycler hybridisation probe system is based on two oligonucleotide probes that hybridise, in a head-to-tail arrangement, to adjacent sequences of the target DNA. Each probe is labelled with a different fluorophore, the first one with Fluorescein at the 3'end (donor), the second one with either LC610,LC640,LC670 or LC705 at the 5'end (acceptor). Additionally, 3'OH terminus of LC probes is phosphorylated to prevent polymerase from extending the primer during PCR reaction. When the fluorophores are in close proximity, there can be a fluorescence resonance energy transfer (FRET) between them, which allows a specific and quantitative real time detection of the template amplification. To avoid sterical problems between the 3' Fluo and 5' LC labels of the probes, there should be a spacer of 1 to 5 nucleotides between the two probes. Net prices, minimal amounts / offered scale of synthesis of LightCycler probes: 1 For oligonucleotides > 33 bases price for each extra base is 20 PLN. 2 Values calculated for 3 pmol of probe per assay (range of optimal amount 2-10 pmol). * Price includes HPLC purification and MALDI ToF quality check to provide probes of exceptional purity. * LightCycler® is a trademark of Idaho Technology Inc. Custom synthesis of LC probes and oligonucleotides containing LCRed dyes under licence of Roche Diagnostics. * Sold under license from Hoffmann La-Roche Ltd. All products are for research only. RNA oligonucleotidesOur offer includes the synthesis of labelled and standard RNA oligos. We have available many types of modifications, both at the 5 ', 3' ends and internal. Please contact us for specific information and pricing for individual RNA modifications. RNA oligonucleotides are HPLC purified. Offered delivered quantities and the prices of RNA oligonucleotides synthesis up to 40nt:
* RNA oligonucleotides longer than 40nt - please contact us * A higher scale of synthesis - please contact us | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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